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DIY CRISPR Kits, Learn Modern Science By Doing
If you had access to modern synthetic biology tools, what would you create?
Josiah Zayner
San Francisco, United States
$71,496 USD total funds raised
333% funded on December 8, 2015

You can still Pre-Order Kits at
http://www.the-odin.com/diy-bacterial-crispr-kit/



Update

I have had a lot of questions about whether anyone can use these kits or how much knowledge or experience or equipment is required. I want to say that everyone will be able to use these kits(they contain everything you need, no extra equipment is required), even if you have had zero experience with Biotechnology(there will be extensive written protocols and videos available). I believe that the only way that this works is if Science is democratized so everyone has access.

The ODIN(http://the-odin.com ) was started on the premise that if the Scientific population of the world doubled or tripled, it would change everything

-Therapeutics and medicine - materials technology
-Fuel and food
-What if we could compost synthetic plastics?

Synthetic Biology is not illegal
So what is stopping this from happening?

Until now, no one has taken the time to develop protocols and methods and then be willing to provide all of this for a reasonable price that can be afforded without large institutional grants.



Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR) is just a long name to say that Scientist found a protein(Cas9) that can use an RNA guide to make highly specific cuts in DNA. This allows unprecedented abilities to edit and engineer DNA. The reason it is such a great Synthetic Biology tool is the specificity and general applicability. One can target almost any DNA region in almost any organism and the time to do this is an order of magnitude less than before with other genetic engineering techniques.

What if you could learn about new cutting edge techniques like CRISPR by actually performing experiments using them!


*Note to BioHackers: Each Kit comes with all sequence and cloning detail so you can perform your own custom genome engineering.


Featured In



CRISPR Based Kits

Bacteria are a commonly used organism in Synthetic Biology because they grow fast and have simple cellular structures, making them easy to engineer. This kit makes specific edits to genes using CRISPR allowing the bacteria to survive on special growth media when it normally would not. Everything required to perform these experiments is included in the kit.

Sample CRISPR Kit Contents Include but are not limited to: A 20-200 uL professional lab grade micropipette, pipette tips, a microcentrifuge tube rack and tubes, plates and media, DNA and yeast or bacterial strains.


Yeast are a commonly used organism in Synthetic Biology because they are one of the simplest Eukaryotes (their cells are similar to mammals like Humans!). Normally, yeast grow with a nice creamy white color. This kit makes specific edits to the ADE2 gene using CRISPR. This causes red pigment to accumulate and the yeast to turn red. Yeast require a more complex media to grow so this kit is more expensive. Everything required to perform these experiments is included in the kit.




Synthetic Biology Non-CRISPR Based Kits


Light Controlled Bacteria Kit

Have you ever wanted a remote control to the genetics inside a living cell? Well now you can have that with the Light Controlled Bacteria Kit. Using this Kit you will learn about optogenetic engineering techniques and fluorescence and how to control the genetics of bacteria using blue light!



Engineering Glowing Bacteria Kit

What if you could engineer the genetics of a bacteria to make them glow and give off light? This kit teaches you about genetic engineering and Bioluminescence so you can create your own glowing bacteria.


What would you create?



My name is Josiah Zayner. I received my Ph.D. in Molecular Biophysics from the University of Chicago studying protein engineering. I have spent the past two years as a Research Fellow in NASA’s Synthetic Biology program where I work on engineering bacteria for terraforming Mars. Budget and funding issues  at NASA necessitate that I mostly work alone. I figured there has to be another way where more people can contribute to Science. For this reason, I started The ODIN about a year ago to provide resources to BioHackers who wanted to do Science at home. In my free time I have been teaching BioHacking classes and testing protocols to build these kits. Now I just need you! Without you ,Science will remain the stagnant behemoth out of everyone’s reach. With you, people will be able to contribute to solving some of the most pressing issues we face in health, medicine, food and fuel. If we work together we can Create Something Beautiful.

When not sleeping or performing cutting edge Science, James Baxter(Orange Cat) and Trypsin(Grey Cat) enjoy eating, snuggling and chasing each other.



FAQ

Ohhemmm gee, is everyone going to kill themselves with these kits?

There is nothing in these kits that is harmful to your health besides maybe the shot glasses. The Bacteria are less harmful than bacteria on your skin and the Yeast are almost identical to the one you use when cooking.


What about a zombie apocalypse?

Though CRISPR can work in a variety of organisms specific changes would need to be made to the DNA in order for it cross any species barrier. The only zombies you will likely see will be your friends from using our shot glasses too much. Isn’t the new season of The Walking Dead much better though?


If you could be any super hero who would it be?

Either Nightcrawler from X-Men or Iron Man.


Select a Perk
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Biohacker Stickers
Our cool Biohacker logo, Stickers that say "Create Something Beautiful" and "BioHack the Planet" for you to stick anywhere(we recommend most places except for faces of friends).
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March 2016
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Shot Glass
A Shot Glass Inscribed with "Create Something Beautiful" on the front and "BioHack the Planet" on the back.
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February 2016
3 out of 100 claimed
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$20
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Biohacker T-shirt
BioHack the PLAANNEETTT! See t-shirt design in campaign summary
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Estimated delivery
March 2016
9 out of 100 claimed
Ships Worldwide
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$75
USD
+ Shipping
Make Glow in the Dark Bacteria
Put some DNA into bacteria that makes them glow in the dark, fun for the whole family. This kit contains everything you need to engineer glowing bacteria. Shipping might not be available to all locations, please check with local laws before ordering.
Read More
Estimated delivery
March 2016
26 out of 50 claimed
Ships Worldwide
Get this perk
$75
USD
+ Shipping
Light Controlled Bacteria
Have you ever wanted to control the genetics of bacteria using just blue light? Well now you can. A synthetic biology circuit developed by my favorite Germans allows you to turn on and off a gene that makes bacteria look red(RFP). This kit contains everything you need to engineer some bacteria to have a light controlled gene circuit. Shipping might not be available to all countries, please check with local laws before ordering.
Read More
Estimated delivery
March 2016
5 out of 50 claimed
Ships Worldwide
Get this perk
$100
USD
+ Shipping
Light Control Advanced
Have you ever wanted to control the genetics of bacteria using just blue light? This kit contains everything you need to engineer some bacteria to have a light controlled gene circuit. It also contains restriction enzymes and DNA ligase so that you can put your own genes under the control of light! Shipping might not be available to all locations, please check with local laws before ordering.
Read More
Estimated delivery
March 2016
12 out of 20 claimed
Ships Worldwide
Get this perk
$130
USD
+ Shipping
Bacteria DIY CRISPR Kit #3
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering? This kit includes everything you need to make precision genome edits in bacteria at home including Cas9, tracrRNA, crRNA and Template DNA template for an example experiment. Includes example experiment to make a genome mutation(K43T) to the rpsL gene changing the 43rd amino acid, a Lysine(K) to a Threonine(T) allowing the bacteria to survive on Strep media. Enough for 5+ experiments.
Read More
Estimated delivery
July 2016
2 out of 100 claimed
Ships Worldwide
Get this perk
$160
USD
+ Shipping
Yeast DIY CRISPR Kit #2
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering. This kit includes everything you need to make precision genome edits in yeast at home including Cas9, gRNA and Donor DNA template for an example experiment. Item contents include but are not limited to: a laboratory grade pipette, media and plates, yeast and DNA. Shipping might not be available to all countries, please check with local laws before ordering.
Read More
Estimated delivery
May 2016
37 out of 100 claimed
Ships Worldwide
Get this perk
$450
USD
+ Shipping
All the Kits Belong to You #2
You will receive one of each pre-stretch goal kit(Glow in the dark bacteria, light controlled bacteria, CRISPR for bacteria and yeast), a t-shirt and two shot glasses(because Scientist shouldn't drink alone unless they are drinking doubles).
Read More
Estimated delivery
June 2016
24 out of 30 claimed
Ships Worldwide
Get this perk
$500
USD
+ Shipping
Classroom Kits
Choose either our Glow in the Dark Bacteria or Light Controlled Bacteria Kits and we will send you a custom version that has enough for a classroom of 20! This is not the same as 20 of the normal kits.
Read More
Estimated delivery
April 2016
0 out of 10 claimed
Ships Worldwide
Get this perk
$3000
USD
Your Own Genetics Lab
WHATTTT! I know crazy right? We will set you up with everything you need to start your own extensive home lab to do synthetic and molecular biology (pipettes, centrifuge, PCR machine, gel electrophoresis, reagents, chemicals, &c.). We will guide you through setting it up and we will also provide you with a CRISPR kit and other kits to get you started! Available only in the US unless you are willing to pay much extra for shipping fees. Please contact us with any questions about contents.
Read More
Estimated delivery
June 2016
4 out of 5 claimed
Ships to: United States
Get this perk
$5000
USD
Create Your Own Organism
We will work with you to edit the genome of a bacteria or yeast to contain a unique desired trait for your own, personal, original, unique, genetically engineered organism. We can set you up to do the experiments yourself or we can do it all for you! Please contact us beforehand if you have any questions on what is possible.
Read More
Estimated delivery
August 2016
2 out of 5 claimed
Ships Worldwide
Get this perk
SOLD OUT
$130
USD
+ Shipping
Bacteria DIY CRISPR Kit
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering. This kit includes everything you need to make precision genome edits in bacteria at home including Cas9, gRNA and Donor DNA template for an example experiment. Item contents include but are not limited to: a laboratory grade pipette, media and plates, bacteria and DNA. Shipping might not be available to all locations, please check with local laws before ordering.
Read More
Estimated delivery
March 2016
52 out of 50 claimed
Ships Worldwide
Get this perk
SOLD OUT
$130
USD
+ Shipping
Bacteria DIY CRISPR Kit #2
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering. This kit includes everything you need to make precision genome edits in bacteria at home including Cas9, gRNA and Donor DNA template for an example experiment. Item contents include but are not limited to: a laboratory grade pipette, media and plates, bacteria and DNA. Shipping might not be available to all countries, please check with local laws before ordering.
Read More
Estimated delivery
April 2016
54 out of 50 claimed
Ships Worldwide
Get this perk
SOLD OUT
$160
USD
+ Shipping
Yeast DIY CRISPR Kit
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering. This kit includes everything you need to make precision genome edits in yeast at home including Cas9, gRNA and Donor DNA template for an example experiment. Item contents include but are not limited to: a laboratory grade pipette, media and plates, yeast and DNA. Shipping might not be available to all locations, please check with local laws before ordering.
Read More
Estimated delivery
March 2016
51 out of 50 claimed
Ships Worldwide
Get this perk
SOLD OUT
$450
USD
+ Shipping
All the Kits Belong to You
You will receive one of each pre-stretch goal kit(Glow in the dark bacteria, light controlled bacteria, CRISPR for bacteria and yeast), a t-shirt and two shot glasses(because Scientist shouldn't drink alone unless they are drinking doubles).
Read More
Estimated delivery
May 2016
10 out of 10 claimed
Ships Worldwide
Get this perk
Select a Perk
$6
USD
Biohacker Stickers
Our cool Biohacker logo, Stickers that say "Create Something Beautiful" and "BioHack the Planet" for you to stick anywhere(we recommend most places except for faces of friends).
Read More
Estimated delivery
March 2016
12 out of 500 claimed
Ships Worldwide
Get this perk
$12
USD
+ Shipping
Shot Glass
A Shot Glass Inscribed with "Create Something Beautiful" on the front and "BioHack the Planet" on the back.
Read More
Estimated delivery
February 2016
3 out of 100 claimed
Ships Worldwide
Get this perk
$20
USD
+ Shipping
Biohacker T-shirt
BioHack the PLAANNEETTT! See t-shirt design in campaign summary
Read More
Estimated delivery
March 2016
9 out of 100 claimed
Ships Worldwide
Get this perk
$75
USD
+ Shipping
Make Glow in the Dark Bacteria
Put some DNA into bacteria that makes them glow in the dark, fun for the whole family. This kit contains everything you need to engineer glowing bacteria. Shipping might not be available to all locations, please check with local laws before ordering.
Read More
Estimated delivery
March 2016
26 out of 50 claimed
Ships Worldwide
Get this perk
$75
USD
+ Shipping
Light Controlled Bacteria
Have you ever wanted to control the genetics of bacteria using just blue light? Well now you can. A synthetic biology circuit developed by my favorite Germans allows you to turn on and off a gene that makes bacteria look red(RFP). This kit contains everything you need to engineer some bacteria to have a light controlled gene circuit. Shipping might not be available to all countries, please check with local laws before ordering.
Read More
Estimated delivery
March 2016
5 out of 50 claimed
Ships Worldwide
Get this perk
$100
USD
+ Shipping
Light Control Advanced
Have you ever wanted to control the genetics of bacteria using just blue light? This kit contains everything you need to engineer some bacteria to have a light controlled gene circuit. It also contains restriction enzymes and DNA ligase so that you can put your own genes under the control of light! Shipping might not be available to all locations, please check with local laws before ordering.
Read More
Estimated delivery
March 2016
12 out of 20 claimed
Ships Worldwide
Get this perk
$130
USD
+ Shipping
Bacteria DIY CRISPR Kit #3
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering? This kit includes everything you need to make precision genome edits in bacteria at home including Cas9, tracrRNA, crRNA and Template DNA template for an example experiment. Includes example experiment to make a genome mutation(K43T) to the rpsL gene changing the 43rd amino acid, a Lysine(K) to a Threonine(T) allowing the bacteria to survive on Strep media. Enough for 5+ experiments.
Read More
Estimated delivery
July 2016
2 out of 100 claimed
Ships Worldwide
Get this perk
$160
USD
+ Shipping
Yeast DIY CRISPR Kit #2
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering. This kit includes everything you need to make precision genome edits in yeast at home including Cas9, gRNA and Donor DNA template for an example experiment. Item contents include but are not limited to: a laboratory grade pipette, media and plates, yeast and DNA. Shipping might not be available to all countries, please check with local laws before ordering.
Read More
Estimated delivery
May 2016
37 out of 100 claimed
Ships Worldwide
Get this perk
$450
USD
+ Shipping
All the Kits Belong to You #2
You will receive one of each pre-stretch goal kit(Glow in the dark bacteria, light controlled bacteria, CRISPR for bacteria and yeast), a t-shirt and two shot glasses(because Scientist shouldn't drink alone unless they are drinking doubles).
Read More
Estimated delivery
June 2016
24 out of 30 claimed
Ships Worldwide
Get this perk
$500
USD
+ Shipping
Classroom Kits
Choose either our Glow in the Dark Bacteria or Light Controlled Bacteria Kits and we will send you a custom version that has enough for a classroom of 20! This is not the same as 20 of the normal kits.
Read More
Estimated delivery
April 2016
0 out of 10 claimed
Ships Worldwide
Get this perk
$3000
USD
Your Own Genetics Lab
WHATTTT! I know crazy right? We will set you up with everything you need to start your own extensive home lab to do synthetic and molecular biology (pipettes, centrifuge, PCR machine, gel electrophoresis, reagents, chemicals, &c.). We will guide you through setting it up and we will also provide you with a CRISPR kit and other kits to get you started! Available only in the US unless you are willing to pay much extra for shipping fees. Please contact us with any questions about contents.
Read More
Estimated delivery
June 2016
4 out of 5 claimed
Ships to: United States
Get this perk
$5000
USD
Create Your Own Organism
We will work with you to edit the genome of a bacteria or yeast to contain a unique desired trait for your own, personal, original, unique, genetically engineered organism. We can set you up to do the experiments yourself or we can do it all for you! Please contact us beforehand if you have any questions on what is possible.
Read More
Estimated delivery
August 2016
2 out of 5 claimed
Ships Worldwide
Get this perk
SOLD OUT
$130
USD
+ Shipping
Bacteria DIY CRISPR Kit
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering. This kit includes everything you need to make precision genome edits in bacteria at home including Cas9, gRNA and Donor DNA template for an example experiment. Item contents include but are not limited to: a laboratory grade pipette, media and plates, bacteria and DNA. Shipping might not be available to all locations, please check with local laws before ordering.
Read More
Estimated delivery
March 2016
52 out of 50 claimed
Ships Worldwide
Get this perk
SOLD OUT
$130
USD
+ Shipping
Bacteria DIY CRISPR Kit #2
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering. This kit includes everything you need to make precision genome edits in bacteria at home including Cas9, gRNA and Donor DNA template for an example experiment. Item contents include but are not limited to: a laboratory grade pipette, media and plates, bacteria and DNA. Shipping might not be available to all countries, please check with local laws before ordering.
Read More
Estimated delivery
April 2016
54 out of 50 claimed
Ships Worldwide
Get this perk
SOLD OUT
$160
USD
+ Shipping
Yeast DIY CRISPR Kit
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering. This kit includes everything you need to make precision genome edits in yeast at home including Cas9, gRNA and Donor DNA template for an example experiment. Item contents include but are not limited to: a laboratory grade pipette, media and plates, yeast and DNA. Shipping might not be available to all locations, please check with local laws before ordering.
Read More
Estimated delivery
March 2016
51 out of 50 claimed
Ships Worldwide
Get this perk
SOLD OUT
$450
USD
+ Shipping
All the Kits Belong to You
You will receive one of each pre-stretch goal kit(Glow in the dark bacteria, light controlled bacteria, CRISPR for bacteria and yeast), a t-shirt and two shot glasses(because Scientist shouldn't drink alone unless they are drinking doubles).
Read More
Estimated delivery
May 2016
10 out of 10 claimed
Ships Worldwide
Get this perk
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